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Zhang Yue,He Yinfeng,Gu Yue,Wang Yan,Zheng Yanxue.Stress resistance and antioxidant properties of lactic acid bacteria with high biofilm production[J].Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE) ,2021,37(6):282-288
Stress resistance and antioxidant properties of lactic acid bacteria with high biofilm production
Received:October 13, 2020  Revised:March 09, 2021
Foundation item:国家自然科学基金项目(31960467);内蒙古自治区自然科学基金项目(2019BS03002);内蒙古自治区研究生科研创新资助项目(B20191147Z)
Author NameAffiliation
Zhang Yue Department of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China 
He Yinfeng Department of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China 
Gu Yue Department of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China 
Wang Yan Department of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China 
Zheng Yanxue Department of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China 
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Abstract: Most bacteria in the natural environment choose to live in the membrane state, due mainly to better advantage over the planktonic state. The resistance of strains to the external environment can be significantly improved, when lactic acid bacteria behave in the form of a membrane state. Therefore, it is a benefit to investigate the stress resistance of strains in biofilm state for the production mechanism behind the lactic acid bacteria biofilm under environmental stress. In this study, two strains of Pediococcus acidilactici RJ2-1-4, TG1-1-10 and two strains of Lactobacillus plantarum RJ1-1-4, RM1-1-11 (They were both high-yield biofilm strains) were selected to systematically explore the tolerance of floating and membranous strains to acid, alkali, bile salt, simulated artificial gastrointestinal fluid, and antioxidant ability. The results showed that the growth of strain was inhibited under the condition of extremely acid, but the growth of membrane state RM1-1-11 at pH 3.0 was significantly higher than that of the planktonic state (P<0.05). The density of bacteria increased with the increase of pH value, whereas, the alkaline environment in pH 7.0-9.0 inhibited the growth of three strains except TG1-1-10. The growths of membranous strains RJ2-1-4 and G1-1-10 were significantly lower than those of floating state (P<0.05), particularly that the growth of strain increased slightly, when the concentration of bile salt was 0-0.03%. However, the growth of strain was inhibited as the concentration of bile salt continued to increase. In addition, the TG1-1-10 growth of planktonic strain was higher than that of membrane strain, whereas the growth of the other three strains in the membrane state was significantly higher than those of floating strain. After the strains were treated in the simulated artificial gastrointestinal fluid for 3 hours, it was found that the survival rate of membrane strains in the gastric and intestinal juices improved, compared with the planktonic strains. There was a certain scavenging ability of four strains for different kinds of free radicals. The scavenging rates were ranked in order: HO·>DPPH·> lipid peroxidation > superoxide anion. Specifically, the clearance rate of RJ2-1-4 floating bacteria suspension on DPPH· was 214.12 μg/mL, while the clearance rate of RJ2-1-4 floating CFS to superoxide anion was 93.8 μg/mL, and the clearance rates of RJ2-1-4 coated CFS and TG1-1-10 floating CFS on HO· were 713.81 μg/mL and 637.01 μg/mL, respectively. The clearance rate of lipid peroxides by RM1-1-11 biofilm suspension was 122.82 μg/mL. Lactic acid bacteria in biofilm state had certain protective effects on acid, alkali, bile salt and artificial gastrointestinal fluid, but there was specificity among strains, even in the same genus. The anti-oxidation ability of membrane strain was higher than that of planktonic state, but there was a definite difference in different kinds of free radicals. The finding can provide a significant support to a further investigation on the resistance of lactic acid bacteria to environmental stress in biofilm states.
KeyWord:bacteria  antioxidation  biofilm  lactic acid bacteria  stress resistance
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